Appendix A: Planning & Community Engagement
-
Specific permits are required at the Federal, State, and County levels. ↗️ See this page for the most up-to-date table.
-
Flyers with QR code linking to a survey and more information help spread awareness and gather community opinions about the project.
-
RWR distributes informational flyers advertising Hana Pūkoʻa events, where community members help prepare corals for different stages of the restoration process.
-
The RWR team solicits and briefs volunteers using printed and digital materials as well as live events. This flyer advertises a Core Volunteer training event, and a sample registration form is provided below.
A sample Eventbrite page can also be found here. -
RWR designed a flyer explaining the restoration process to increase community awareness of the project.
Appendix B: Restoration & Monitoring
-
A nursery platform, or table, is a secure substrate that serves as an interim location for corals to acclimate and grow in the field. Not all projects require a nursery table.
Please download the notes on ⬇️ Developing a Nursery Platform for more information.
-
These activities can be completed with the help of community volunteers.
-
Biopsy preparation entails collecting a small sample of live tissue, or biopsy, from each COO and mounting it onto a labeled aragonite plug, which is then transported to stress testing tanks – either at a facility or a portable Coral Bleaching Automated Stress System (CBASS).
-
To identify which collected COO were more thermally resilient, the Coral Resilience Lab (CRL) at HIMB created a stress-testing system designed to mimic the natural environment, test individuals from more than one species simultaneously, and produce results quickly.
The CRL design uses heating and cooling reservoir tanks, solenoids, and computer programming to mimic daily temperature cycles with a gradual overall temperature increase (bleaching) and decrease (recovery). Researchers run this bleaching/recovery profile until roughly half of the biopsies from all coral species bleach or die (about three weeks), at which point they plot results on a “bleaching curve” to distinguish more resilient corals (those that did not bleach or took longer to bleach) from less resilient corals (those that bleached or died).
⬇️ Download the full protocol here.
↗️ Reference Caruso et al., 2021 for more background on these techniques.
-
Fragmentation is a natural method of asexual coral reproduction wherein a coral colony is broken up into smaller (about “double thumb-sized”) pieces. This method is different from micro-fragmentation, which requires slicing coral into very small pieces, growing it across an artificial hard surface, and outplanting it along with the artificial substrate. In coral restoration, fragmentation has been shown to enhance growth rates. Some projects outplant the fragments at random to increase dispersion of resilient individuals across a location. Another option is to outplant fragments from the same colony close together to encourage fusion of the clones. This technique creates a larger colony faster than would otherwise be possible.
-
In the outplanting phase, resilient coral fragments, still mounted on aragonite plugs, are transported to a chosen outplanting site and transferred from storage racks onto suitable substrates.
-
Coral colors measured against a color reference card can be used to assess COO and outplant status.
Visit the ↗️ Coral Reef Ecology Lab for more information and training on the Hawaiian Koʻa Card.
-
Photogrammetry creates digital archives of the reef status and captures higher quality information – such as community composition, reef and coral geometric complexity, outplant mortality, growth, and survivorship – than traditional survey methods. Software such as Agisoft Metashape Professional Edition and AddTools for Metashape plugin can help with processing photogrammetry data and used to measure growth, survivorship, and success of outplants over time.
-
Sample datasheet structure for the biopsy and fragmenting steps can be found below.
Biopsying Corals of Opportunity
a) Datasheet example for station 1: tagging, species and photo identification, and color health score using the Koʻa card
b) Datasheet example for station 2: tracking COO biopsies (2 biopsies in this case) prior to the stress test
Fragmenting Resilient Corals
[Image: Fragmenting Datasheets]
a) Datasheet for station 1: COO tag identification, colony size before fragmentation, species identification, color health score using the ko‘a card, and total number of fragments processed from each COO
b) Datasheet for station 2: tracking the amount of fragments, fragment size, and rack number from each COO processed
Map: RWR Project Sites Referenced
Appendix C: Maui Nui Site Selection
-
Adapted from Kuleana Coral Restoration SITE RECON lesson
Aggregate reef: Hard-bottom substrate with corals, also referred to as continuous of consolidated reef. Most reefs that do not fall in other types are recorded as aggregate reef.
Pavement: Flat, low-relief, solid rock in broad areas often with partial coverage of sand, algae, hard coral, gorgonians, zooanthids, or other sessile invertebrates that are dense enough to begin to obscure the underlying surface.
Spur and groove: habitat with alternating sand and coral formations that are oriented roughly perpendicular to the shore, bank, or shelf.
Rock/boulder: Large, irregularly shaped carbonate blocks or boulders or volcanic rock often extending offshore from the island bedrock or headlands.
Reef rubble: Unconsolidated small (<10cm) fragments of coral skeletons or reef rock often colonized with filamentous or other macroalgae.
Aggregate patch reefs: Coral formations that are isolated from other coral reefs formations by sand or other habitats and that have no organized structural axis relative to the shore or shelf edge covering > 10% of the survey.
Pavement with patch reefs: Areas of pavement with occasional patch reefs formations that make up less than 10% of the general area.
Pavement with sand channels: Habitats of pavement with alternating sand/surge channel formations that are perpendicular to the shore, bank, or shelf.
Sand with scattered coral/rock: Sand bottom with scattered rocks or small, isolated coral heads that make up <10% of the total area.
-
Reef Rubble
Aggregate Reef
Aggregate Patch Reefs
Pavement with patch reefs/rubble
Spur and groove
Some pavement (harder to find)
The reefs of South Maui are much younger than the reefs on Oʻahu. The island of Oʻahu is 2-3 million years older than Haleakalā, the summit above South Maui’s reefs. The reefs on Oʻahu, therefore, have had millions of years more to develop and grow than Maui’s reefs, providing older, larger, and more abundant pavement-type habitats for restoration.
Work in South Maui was proposed due to the existing work occurring mauka in Kula moku: fencing for ungulate control, sediment removal from flood-prone gulches, wetland restoration, and reef-friendly landscaping. While many shallow reefs in Kula Kai (North Kīhei) are declining due to sedimentation, many resilient reefs still exist at depths around 30-35 feet, indicating that restoration could be a useful tool for the area.
Māʻalaea Bay as a whole, for example, has seen reduced turbidity since the creation of Keālia Pond National Wildlife Refuge and the areas along the pond have some of the best water quality recorded by Hui O Ka Wai Ola. Water quality seems to be improving moving from Kīhei towards Māʻalaea. Additionally, some pavement substrate exists between 18 and 24 feet in Māʻalaea Bay, along Sugar Beach.
Shallower habitat substrate in Kula Kai/North Kīhei consists of reef rubble, aggregate reef, and aggregate patch reefs.
The pavement habitat type is ideal for restoration activities utilizing large rescued colonies (LRC) and module outplanting. While some of it exists in South Maui, it is less common in Kula Kai/Kula moku.
While sedimentation is common in Kula Kai, some offshore areas are outside the usually turbid zones and show high coral cover and species diversity. These areas are accessible by boat, around 30-35 feet in depth, and have restoration potential.
One of the most helpful parts of learning about site selection and habitat types was diving with Kuleana Coral on Oʻahu and training in their restoration techniques. In-person training and visuals are highly effective in understanding what to look for in terms of habitat and reef type, rather than relying on maps or bathymetric data. Diving with existing coral restoration organizations is highly recommended to see the “live” substrates required for different restoration activities.